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2 edition of Studies on the haemolysis of mammalian erythrocytes found in the catalog.

Studies on the haemolysis of mammalian erythrocytes

Malcolm F. Coldman

Studies on the haemolysis of mammalian erythrocytes

thesis presented for the degree of Doctor of Philosophy in the University of London.

by Malcolm F. Coldman

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  • 31 Currently reading

Published by Coldman in Bradford .
Written in English


Edition Notes

Typescript.

The Physical Object
Pagination89p.
Number of Pages89
ID Numbers
Open LibraryOL13654214M

  Haemolysis of infected erythrocytes also triggers the formation of P. falciparum gametocytes. The treatment of the malaria parasite with drugs such as steroid hormones [ 10 ], fansidar [ 11 ] and chloroquine or sulfadoxine-pyrimethamine [ Cited by: 1.   In general, lysis is the destruction of cells by a loss of membrane integrity. The intracellular contents are then released from the cells into the medium in which the cells were dissolved. Hemolysis specifically, is the lysis of red blood cells .

A blood cell, also called a hematopoietic cell, hemocyte, or hematocyte, is a cell produced through hematopoiesis and found mainly in the types of blood cells include; Red blood cells (erythrocytes); White blood cells (leukocytes); Platelets (thrombocytes); Together, these three kinds of blood cells add up to a total 45% of the blood tissue by volume, with the remaining . Haemolysis definition: the disintegration of red blood cells, with the release of haemoglobin, occurring in a | Meaning, pronunciation, translations and examples.

erythrocytes exceeds cells. In periods of increased erythrocyte loss due to haemolysis or hemorrhage, the production of erythrocytes increases rapidly and markedly. However, an overproduction of erythrocytes does not occur even after the most severe loss of erythrocytes. In haematopoiesis, a few rare hematopoietic stem cells in the.   Instead, erythrocytes depend on the anaerobic conversion of glucose by the Embden-Meyerhof pathway for the generation and storage of high-energy phosphates. Moreover, erythrocytes possess a unique glycolytic bypass for the production of 2,3-bisphosphoglycerate (2,3-DPG), the Rapoport-Luebering by:


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Studies on the haemolysis of mammalian erythrocytes by Malcolm F. Coldman Download PDF EPUB FB2

The values of the activation parameters of haemolysis for TABLE 1 The activation parameters of malonamide-induced haemolysis of mammalian erythrocytes at constant osmotic concentration ( atm, 20) AH* AS* AV* AE* Animal species kcal mol-1 cal deg mol-1 1 mol-1 kcal mol Cat Rat Sheep Pig Author: W.

Goon. Thermohaemolysis of mammalian erythrocytes might contribute to the quantitative differences in thermohaemolysis of different species.

This assump- tion was substantiated by the following result. The thermoresistivity of cells is defined as the time needed to reach 50% haemolysis in NaCI saline at constant by: Haemolysis of human, rabbit, cow, hamster, guineapig, pig and sheep erythrocytes was not observed in solutions of % or more of glucose.

Except for sheep, human and dog erythrocytes, haemolysis was depressed in rate but not completely prevented by phosphate-buffer solution of Cited by: In vitro, L-sorbose induces in dog erythrocytes haemolysis which is dependent upon time of incubation, temperature, pH and the concentration of sorbose.

The in vitro susceptibility to sorbose-induced haemolysis is different in various by: 4. This paper, which is of a technical nature, must be read in the original by those interested. The following are the conclusions: " Our results suggest that differences in the susceptibility of various types of erythrocytes to haemolysis with hypotonic solution of sodium chloride are due largely to differences in form and not to differences in osmotic by:   To evaluate why hemolysis of red blood cells (RBC) by bile acids varies in different mammalian species, we determined the mean corpuscular volume (MCV), lipid content and the concentrations of the conjugates of deoxycholate and of NaCl inducing 50% hemolysis of RBC from healthy humans, pigs, horses, cows, sheep and jaundiced humans.

A volume of Cited by: Although many studies have shown saponin as a haemolytic agent, but these were in vitro studies [4, 6,12]. Bissinger et al. [6] proposed that the mechanism of haemolysis of. Red blood cells (RBCs), also referred to as red cells, red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek erythros for "red" and kytos for "hollow vessel", with -cyte translated as "cell" in modern usage), are the most common type of blood cell and the vertebrate's principal means of delivering oxygen (O 2) to the body tissues—via blood flow through the FMA: Measuring osmosis and hemolysis of red blood cells Article in AJP Advances in Physiology Education 41(2) June with 2, Reads How we measure 'reads'.

Hemolysis of rabbit erythrocytes by purified staphylococcal alpha-toxin: I. Kinetics of the lytic reaction. Bacteriol. – —The hemolytic activity of purified staphylococcal alpha-lysin was found to be directly proportional to toxin concentration and inversely related to the log concentration of rabbit erythrocytes.

Activity Cited by: erythrocytes instead of ATP produced from mitochondria or by anaerobic respiration. The camels evolved elliptical erythrocytes and kept them, by retaining the MBs.

The abortion of the nucleus occurs and, nevertheless, the biconcave disk cannot form. The bilaminar, oval wafer is low in volume, about double in S/V, rich in Hb, and the many thin File Size: KB. The intracellular fluid of erythrocytes is a solution of salts, glucose, protein and hemoglobin.

A % NaCl solution is said to be isotonic: when blood cells reside in such a medium, the intracellular and extracellular fluids are in osmotic equilibrium across the cell membrane, and there is no net influx or efflux of water.

Strains of Yersinia enterocolitica serotype 03 produce a distinct β-haemolysis on bovine blood agar [Abstr. Hyg.55, abstr. This haemolytic substance was released from the bacterial cells by sonic disintegration. "The substance was non-dialysable, thermolabile, antigenic, and sensitive to trypsin.

Chromatographic studies indicated a high molecular by: 2. MATSUZAWA T IKARASHI Y, Haemolysis of various mammalian erythrocytes in sodium chloride, glucose and phosphate-buffer solutions.

Lab. Anim.13 pp. [] COLDMAN MF, GENT M, GOOD W, The osmotic fragility of mammalian erythrocytes in hypotonic solutions of sodium chloride, Comp. Biochem. Background. InGorter and Grendel were the first to report the bilayer nature of the cell structure of the cell membrane was further advanced by the work of Singer and Nicolson (), who described the presence and location of proteins in the bilayer and developed the fluid mosaic the mammalian cell membrane, the phospholipid bilayer alone is Cited by: 2.

haemolysis, hemolysis, haematolysis or hematolysis n, pl -ses (-ˌsiːz) (Biology) the disintegration of red blood cells, with the release of haemoglobin, occurring in a living organism or in a blood sample hemolysis, haemolysis the breaking down of erythrocytes with liberation of hemoglobin in the blood.

— hemolytic, haemolytic, adj. See also. Hemolytic and nonhemolytic bacteria were incubated aerobically and anaerobically with the following substrates: erythrocytes, hemoglobin, myoglobin, cytochrome c, hematin, iron hematoporphyrin, copper hematoporphyrin, protoporphyrin, and bilirubin.

After 18 hr at 37 C the evolved CO was measured by gas chromatography. Physiological hemolysis, a process that completes the day life cycle of the erythrocyte, takes place continuously in man and animals. Under normal physiological conditions percent of all the erythrocytes—usually the “aging” ones—undergo lysis.

The final decomposition of these aging erythrocytes takes place primarily in the spleen. which equals 61°C for human erythrocytes [14,15]. This transition enhances ion permeability eliciting colloid-osmotic lysis at constant temperatures [15] and during heating with constant rate [14].

The same mechanism of thermal hemolysis was found in mammalian erythrocytes [7,17]. As for human erythrocytes, the E a.

Glut1 is a member of the mammalian facilitative glucose transporter family Glut (1,2). The kDa integral membrane protein is expressed in all human cells, and is particularly abundant in human erythrocytes, fibroblasts and endothelial cells. In erythrocytes it is responsible for the uptake of glucose—the cell’s major energy Size: 5MB.

Start studying Ch. 17 Blood. Learn vocabulary, terms, and more with flashcards, games, and other study tools.Erythrocytes. The morphology of erythrocytes varies with 2 to± µm in size; appears circular, discoid, central pallor with slight anisocytosis whereas the rouleaux formation() is common in tiger’s anaik, () also reported the mild anisocytosis in physically restrained r, the range and mean (with one standard deviation) of total Cited by: 5.Allow the volumeter knob to return slowly to the up position.

Never permit it to snap up.; Wait one to two seconds to ensure that the full volume has been drawn into the tip (longer for viscous solutions such as blood).